烟草染色体片段代换系的构建与遗传评价
Acta Agronomica Sinica(2016)
Abstract
以综合性状优良的烤烟种质 Y3为轮回亲本,抗烟草黑胫病(0号和1号生理小种)和赤星病的雪茄烟种质Beinhart 1000-1及优质烤烟品种K326为供体亲本,连续回交并结合分子标记辅助选择,构建国内外第一套由256个具有烤烟Y3遗传背景并渐渗有Beinhart 1000-1和K326染色体片段株系代换系群体。该群体携带377个代换片段,分布于烟草24条连锁群上。每个株系携带1~5个代换片段,代换片段长度介于0.05~36.88 cM,平均长度7.75 cM。代换片段重叠累加总长度为2922.57 cM,是烟草基因组总长度的2.61倍。代换片段覆盖总长度为1114.32 cM,烟草基因组覆盖率为99.45%。本研究构建的片段代换系可用于烟草基因定位、复杂性状的QTL分析和标记辅助选择育种。
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Key words
Chromosome segment substitution lines (CSSLs),SSR,Molecular marker assisted selection (MAS),Tobacco (Nicotiana tabacum L.)
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