Kinetics Of The Liposome Adhesion On A Mercury Electrode: Testing Of A Mathematical Model

Liposome adhesion at charged mercury interface is measured by appearance of adhesion signals so-called current transient. The proposed methodology based on new developed mathematical model of consecutive multi-step processes will show how to access kinetic parameters of adhesion process: docking, opening and spreading time constants, respectively. The model was tested using reported current and charge transients of liposome. Model shows agreement with literature value in determination of spreading time constants either by computation from charge or current transients. Contrary, determination of opening time constants shows agreement with computation from current transients only. The fast docking time constant become resolved (at time below the opening time constant), from current transient data and the model of the three-step process. These demonstrate that current and charge transients should be combined in determination of kinetic parameters of adhesion process.