The effect of hydrogen peroxide produced during ultraviolet disinfection of CHO cell culture media

Emma V. Dare,Michelle Gabriel,Afroza Begum,Michael Sasges,Marc G. Aucoin

Process Biochemistry（2017）

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摘要

•Moderate UV can disinfect cell culture media without damage to the media.•H2O2 generated by high UV was investigated as an initiator of deleterious reactions.•UV causes changes beyond what are caused by H2O2 formation.•H2O2 in media only caused changes in pyruvate, acetate, sarcosine and formate.•Catalase addition prevented changes in pyruvate, acetate, sarcosine and formate.

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关键词

Hydrogen Peroxide (PubChem CID: 784),Pyruvate (PubChem CID: 107735),Sarcosine (PubChem CID: 1088),Riboflavin (PubChem CID: 493570),Lumichrome (PubChem CID: 5326566),Thiamine (PubChem CID: 1130),Pyridoxine (PubChem CID: 1054)

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