Effect of two FSH ovarian stimulation treatments on doppler velocimetry and ovarian artery diameter in goats

The aim of this study was to evaluate the effect of two FSH ovarian stimulation treatments on Doppler velocimetry and diameter of ovarian artery. 4 undefined breed goats 4-7 years of age were used. For synchronization of the cycle, intravaginal sponges were inserted containing 60 mg of MAP for 10 days, on the seventh day of treatment 50 ug D-cloprostenol was applied. For ovarian stimulation, the goats were divided into two groups; i) multi-dose (MD) with administration of 120 mg of pFSH intramuscularly, divided into five decreasing doses. Progestogen was administered on the 7th day of the treatment till 24 hours before sponge removal in 12 h intervals; ii) one-shot (OS) pFSH with 70 mg plus 200 IU eCG administered intramuscularly 36 hours before withdrawal of the sponge. Doppler ultrasound scanner apparatus (CTS-8800V, SIUI, China) equipped with linear transrectal probe (6-8 MHz) was used. Goats were maintained in stationed position and urinary bladder was used as a guide, the probe was inserted with the transducer perpendicular to the ventral abdominal wall and scanning was performed at 90° clockwise and 180° counterclockwise for location of the left and right ovaries, ovarian arteries and uterine veins. The examinations were performed before insertion and after removal of sponge to measure the diameter of vascularization in ovarian artery. The angle of insonation was set at 60, and the images were analyzed using the program Image J (National Institutes of Health, USA) previously calibrated (62.3 pixels/cm). Data was presented as mean ± standard deviation and paired-t test was used to assess differences between groups. The visualization of blood flow in ovarian artery in all ultrasound examinations was possible. The End Diastolic Flow (EDF) in the MD and OS group on D0 was 11.20± 1.94 and 13.5± 3.91, respectively. The average EDF on D10 in MD and OS groups was 15.3± 3.91 and 15.5± 5.17 cm/sec, respectively. The average Peak Systolic Velocity (PSV) in MD and OS groups on D0 was 25.6 ± 7.60 and 4.6± 28.5 cm/s, respectively, the same parameter on D10 averaged 27.4± 3.71 for MD group and 29.7± 6.08 cm/s for OS group, with no differences between groups in Doppler parameters (P ˂0.05). The diameter on D0 of the ovarian artery in MD and OS groups was 0.21 ± 0.03 and 00.7 ± 0.17, respectively, whereas at D10, the averages were 0.30 ± 0.04 and 0.22 ± 0.03 in MD and OS groups, respectively. Significant differences (P> 0.05) were observed between days D0 and D10 in the MD group, but there was no difference between groups on D10. In conclusion, the use of two different treatments for ovarian stimulation did not alter the blood irrigation in caprine ovary.