废弃烟梗提取液为基质的产油脂酵母菌的筛选与鉴定
Tobacco Science & Technology(2015)
Abstract
以废弃烟梗水提取液(Tobacco stem extract, TSE)为唯一基质,采用苏丹黑B染色法从市售鲜葡萄中初筛出12株产油脂酵母菌,进一步结合摇瓶发酵实验复筛出2株在TSE中生长良好且油脂产量较高的酵母菌CC820和CA815。对CC820和CA815的菌落、细胞及生理生化特征进行检测, CC820初步鉴定为假丝酵母(Candida sp),CA815初步鉴定为红酵母(Rhodotorula sp)。在接种量5%、装载量60/250 mL、180 r/min和30℃条件下培养5 d,菌株CC820和CA815油脂产量分别为3.53 g/L和3.29 g/L。
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Key words
oleaginous yeasts,fermentation,tobacco,stem
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