Enclosed casting of epoxy resin for rapid fabrication of rigid microfluidic chips

•Epoxy resin was demonstrated for rapidly fabricating rigid microfluidic devices.•Enclosed casting enabled by PDMS intermediate molds can produce ready-to-use devices.•A series of bonding methods were developed for epoxy resin with most common materials.•Multilayer microvalve, PCR, and high-speed flow cytometry imaging were tested.