A single tube system for the detection of Mycobacterium tuberculosis DNA using gold nanoparticles based FRET assay

Amira Mansour,Salma Tammam,Asma Althani, Hassan M.E. Azzazy

Journal of Microbiological Methods（2017）

引用 15|浏览9

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摘要

The global combat against MTB is limited by challenges in accurate affordable detection. In this study, a rapid, affordable, single tube system for detection of unamplified MTB16s rDNA was developed. Utilizing a AuNP based FRET system, this assay achieved a sensitivity and specificity of 98.6% and 90% respectively.

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关键词

Mycobacterium tuberculosis,Fluorescence Resonance Energy Transfer,Gold nanoparticles,16s rDNA,In vitro diagnosis

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