Comparative effectiveness of two different forms of phytoestrogens as a graft material in bony defects

Abstract Purpose To compare the effectiveness of two forms of phytoestrogens in Soybeans as a graft material in bony defects. Material and methods Eighteen bone defects were created in the parietal bones of nine New Zealand white rabbits. Three groups were divided (Six defects each) and filled with either material A in group I, or material B in group II, or left empty as control group. One gm of the purified active fraction obtained from seeds of Glycine max L. (Soybean) Family Leguminosae, rich in isoflavones (Material A) was tested as well as a prepared semi solid pharmaceutical paste (Material B) of the crude bioactive fraction. Defects and surrounding tissue were decalcified and routinely processed for Haematoxylin and Eosin staining. The area percent of newly formed bone was estimated using an image analyzer computer system. Comparison of the results of all groups were carried out.co ANOVA test was used for statistical analysis. Results Regularly arranged, osteoid newly formed bone almost filled the surgical defect in group1, while group 2 revealed partial obliteration of the bony defect with dispersed irregularly arranged bone trabeculae, separated by fibrovascular marrow. Scarce bone trabeculae and wide fibrovascular marrow spaces were observed in the control group. This difference was statistically significant (P  Conclusion The results of this study suggest that phytoestrogens obtained from Soybeans seeds in form of semi solid pharmaceutical paste is more effective than the crude bioactive fraction of the same plant. Further comparative studies are needed on extended time intervals.