A voltammetric study on the interaction between isoproterenol and cardiomyocyte DNA by using a glassy carbon electrode modified with carbon nanotubes, polyaniline and gold nanoparticles

Mikrochimica Acta(2017)

引用 14|浏览17
暂无评分
摘要
An understanding of the mechanism of interaction between the isoproterenol (a β-adrenoreceptor agonist and TAAR1 agonist) and cardiomyocytes is a crucial area of research in order to overcome the side effects of isoproterenol for clinical treatment. The authors describe an electrochemical method to study the effects and response of cardiomyocyte DNA following treatment with isoproterenol. High-purity DNA was isolated from primary cardiomyocytes and used to modify a glassy carbon electrode (GCE) with a nanocomposite of the type DNA/AuNP/PANI/MWCNT (where AuNP stands for gold nanoparticles, PANI for polyaniline, and MWCNT for multiwalled carbon nanotubes). Isoproterenol has a characteristic differential pulse voltammetric peak at approximately 0.38 V (vs. Ag/AgCl), and this signal was used to monitor interactions with DNA double strand with increasing concentrations of isoproterenol. It is shown that DNA is damaged which can be confirmed by detection of DNA damage-related protein expression. The primary mode of binding between isoproterenol and DNA is demonstrated to be intercalation, and the redox reaction is shown to be controlled by adsorption and to be an irreversible single-electron transfer process. Graphical abstract A sensitive electrochemical sensor for the determination of interaction between isoproterenol and cardiomyocyte DNA has been developed by modifying a glassy carbon electrode with a nanocomposite consisting of carbon nanotubes, polyaniline, and gold nanoparticles.
更多
查看译文
关键词
Isoproterenol, Cardiomyocyte, Electrochemical sensor, DNA damage, Drug side effects, Drug-DNA interaction, Cyclic voltammetry, Electrochemical impedance spectroscopy
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要