Chemical Constituents from Mycelia and Spores of Fungus Cordyceps cicadae

Abstract Objective To isolate and identify the chemical constituents from mycelia and spores of the fungus Cordyceps cicadae , respectively. Methods The chemical constituents were isolated and purified by repeated silica gel, Sephadex LH-20, and reversed phase HPLC. The structures of these compounds were elucidated on the basis of extensive spectroscopic analysis, including 1D and 2D NMR. Results Nine known sterols such as ergosterol ( 1 ), ergosterol peroxide ( 2 ), 9,11-dehydroergosterol peroxide ( 3 ), 3β,5α,9α-trihydroxy-(22 E ,24 R )-ergosta-7,22-dien-6-one ( 4 ), 3β,5α,9α,14α-tetrahydroxy-(22 E , 24 R )-ergosta-7,22-dien-6-one ( 5 ), 5α,6α-epoxy-(22 E ,24 R )-ergosta-8(14),22-diene-3β,7α-diol ( 6 ), 3β,5α,6β-(22 E ,24 R )-ergosta-7,22-dien-3,5,6-triol ( 7 ), 3β,5α,6α-6-methoxyergosta-(22 E ,24 R )-7,22-diene-3,5-diol ( 8 ), 4-hydroxy-17 R -methylincisterol ( 9 ), together with a resorcinol derivative, 5- n -nonadecylresorcinol ( 10 ), a cyclodesipeptide, beauvericin ( 11 ), and a nucleoside, N 6 -(2-hydroxyethyl)adenosine ( 12 ) were successively isolated from the cultivated C. cicadae mycelia and spores. Conclusion Compounds 3–10 are reported for the first time from the title sample, beauvericin exhibits significant cytotoxicity against human leukemia cell line and human lung cancer cell line.