家蚕MBP-Bmlp7蛋白的克隆表达及免疫学鉴定
Journal of Shenzhen University Science and Engineering（2017）
Abstract
克隆表达家蚕蚕蛹30 kD(1 kD=1 u)脂蛋白家族成员Bmlp7(Bombyx mori lipoprotein 7)的融合蛋白,鉴定其致敏原性.合成Bmlp7的基因后连接至载体质粒pMAL-C5e上,在大肠杆菌BL21中诱导表达出麦芽糖结合蛋白(maltose binding protein, MBP)-Bmlp7,纯化后,利用Western blot鉴定MBP-Bmlp7融合蛋白与家蚕过敏患者血清中特异性免疫球蛋白E(immunoglobulins E, IgE)的结合能力.结果显示,纯化得到了纯度较高的MBP-Bmlp7融合蛋白,且该融合蛋白能够与家蚕过敏患者血清特异性IgE结合,说明Bmlp7是家蚕中一种潜在的致敏原.
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Key words
immunology,Bombyx mori,30 kD lipoprotein,expression and purification,immunoblotting,fusion protein,immnogenicity
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