Diversity Analysis among Fusarium oxysporum F. Sp. cumini Isolates Using ISSR Markers, Spore Morphology and Pathogenicity

Fusarium oxysporum f. sp. cumini is a serious disease of cumin (Cuminum cyminum L.). Ten isolates of F. oxysporum f. sp. cumini were isolated from major cumin growing districts of Gujarat state to compare molecular methods over traditional for differentiation among the isolates of pathogen. The average 98.70 % of polymorphism was recorded for all the 11 ISSR primers. The polymorphic information content (PIC) value ranged from 0.571 to 0.831 with an average of 0.739 per primer. The IISR primers grouped majority of the isolates based on their geographical location. The microconidia and macroconidia were 0-1 and 2-5 septa respectively and hyaline. All isolates showed varied levels of virulence. The ISSR analysis grouping does not show correlation with spore morphology and pathogenicity of the isolates.