MP26-11 THERAPEUTIC OUTCOMES OF INSULIN-LIKE GROWTH FACTOR-1 RELEASED FROM ALGINATE-GELATIN MICROBEADS ON STRESS URINARY INCONTINENCE IN RATS WITH SIMULATED CHILDBIRTH INJURY

You have accessJournal of UrologyUrodynamics/Lower Urinary Tract Dysfunction/Female Pelvic Medicine: Basic Research & Pathophysiology I1 Apr 2017MP26-11 THERAPEUTIC OUTCOMES OF INSULIN-LIKE GROWTH FACTOR-1 RELEASED FROM ALGINATE-GELATIN MICROBEADS ON STRESS URINARY INCONTINENCE IN RATS WITH SIMULATED CHILDBIRTH INJURY Hao Yan, Liren Zhong, Yaodong Jiang, Jian Yang, Dan Li Lin, Xiaoyi Yuan, Mei Kuang, Anna Rietsch, Emmanuel Opara, Margot Damaser, and Yuanyuan Zhang Hao YanHao Yan More articles by this author , Liren ZhongLiren Zhong More articles by this author , Yaodong JiangYaodong Jiang More articles by this author , Jian YangJian Yang More articles by this author , Dan Li LinDan Li Lin More articles by this author , Xiaoyi YuanXiaoyi Yuan More articles by this author , Mei KuangMei Kuang More articles by this author , Anna RietschAnna Rietsch More articles by this author , Emmanuel OparaEmmanuel Opara More articles by this author , Margot DamaserMargot Damaser More articles by this author , and Yuanyuan ZhangYuanyuan Zhang More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2017.02.782AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Insulin-like growth factor-1 (IGF-1) treatment has been reported to accelerate recovery from stress urinary incontinence (SUI) induced by simulated childbirth injury in rats. However, a local sustained delivery method is ideal for further clinical applications to avoid side effects of IGF-1. The goal of this study was to determine the effects of controlled release of IGF1 from alginate-gelatin microbeads (IGF1-A-G-beads) on sphincter tissue repair in a rat model of stress urinary incontinence (SUI). METHODS Forty-four female Sprague-Dawley rats were divided into 4 equal groups: sham vaginal distension (VD) + saline, VD + saline, VD + empty A-G-beads, & VD + IGF1-A-G-beads). All rats received periurethral injections of A-G-beads immediately after VD. Leak point pressure (LPP) testing and external urethral sphincter (EUS) electromyography (EMG) were performed 1 week later. Urethral tissue and anterior vagina were dissected en bloc for further analysis via histology and immunofluorescence. Quantitative data was analyzed using ANOVA on Ranks followed by a Tukey posthoc test with p < 0.05 indicating a statistically significant difference between groups. Data is presented as mean +/- standard error of the mean. RESULTS LPP was significantly decreased 1 week after VD treated with saline only (23.9 ± 1.3 cmH2O) compared to sham VD (44.4 ± 3.4 cmH2O). LPP was also significantly decreased in the VD + empty A-G-beads group (21.7 ± 0.8 cmH2O) compared to sham VD, demonstrating that the microbeads themselves do not create a bulking or obstructive effect in the urethra. In contrast, rats with VD treated with IGF1-A-G-beads (28.4 ± 1.2 cmH2O) was significantly greater than LPP of rats with VD treated with empty A-G-beads and had LPP partway between and not significantly different from either the sham VD or the VD + saline groups, demonstrating initiation of a reparative effect 1 week after VD. The increase in EUS EMG amplitude with LPP testing was significantly reduced after VD treated with saline or empty A-G-beads compared to sham VD. VD rats treated with IGF1-A-G-beads had EUS EMG amplitude response to LPP testing partway between and not significantly different from either the sham VD or VD + saline groups. Histological analysis demonstrated well-developed, well-organized skeletal muscle fibers in the external urethral sphincter in the VD + IGF1-A-G-beads group, similar to that of sham VD rats. In contrast, substantial muscle fiber attenuation and disorganization was observed in VD rats treated with saline or empty A-G-beads. CONCLUSIONS IGF1-A-G-beads improved recovery in a rat model of SUI, suggesting that these microspheres could provide a local sustained delivery method for IGF-1. © 2017FiguresReferencesRelatedDetails Volume 197Issue 4SApril 2017Page: e323-e324 Advertisement Copyright & Permissions© 2017MetricsAuthor Information Hao Yan More articles by this author Liren Zhong More articles by this author Yaodong Jiang More articles by this author Jian Yang More articles by this author Dan Li Lin More articles by this author Xiaoyi Yuan More articles by this author Mei Kuang More articles by this author Anna Rietsch More articles by this author Emmanuel Opara More articles by this author Margot Damaser More articles by this author Yuanyuan Zhang More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...