MP48-04 EXPRESSION AND FUNCTION OF LYSOPHOSPHATIDIC ACID RECEPTOR 1 IN BLADDER CANCER

You have accessJournal of UrologyBladder Cancer: Basic Research & Pathophysiology II1 Apr 2017MP48-04 EXPRESSION AND FUNCTION OF LYSOPHOSPHATIDIC ACID RECEPTOR 1 IN BLADDER CANCER masao kataoka, tomoyuki koguchi, Michihiro Yabe, Yuuichi Satoh, Nobuhiro Haga, Kei Ishibashi, Ken Aikawa, and Yoshiyuki Kojima masao kataokamasao kataoka More articles by this author , tomoyuki koguchitomoyuki koguchi More articles by this author , Michihiro YabeMichihiro Yabe More articles by this author , Yuuichi SatohYuuichi Satoh More articles by this author , Nobuhiro HagaNobuhiro Haga More articles by this author , Kei IshibashiKei Ishibashi More articles by this author , Ken AikawaKen Aikawa More articles by this author , and Yoshiyuki KojimaYoshiyuki Kojima More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2017.02.1485AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Lysophosphatidic acid (LPA) is one of several physiologically active lipid mediators that promote cell proliferation, cell migration, and cell invasion and are present in serum, ascites, and urine. LPA receptor is considered a potential therapeutic target for treatment of some malignant cancers. Here, we evaluated the expression of LPA receptors, and effect of its receptor expression on recurrence, progression and survival rate in bladder cancer. Furthermore, we examined the bladder cancer invasion mechanism that LPA were associated. METHODS Expression of LPA receptors in bladder cancer specimens from bladder cancer patients (Ta or T1; 49 patients and T2-T4; 17 patients) was examined using real-time PCR and immunohistochemical staining. Matrigel invasion assay, proliferation assay, cell morphological observations were conducted and Rho kinase (ROCK) expression, myosin light chain (MLC) phosphorylation were measured to assess the effects of LPA on T24 cells, which derive from bladder cancer. RESULTS LPA receptor 1 (LPA1) mRNA expression was significantly higher in muscle-invasive bladder cancer (MIBC) specimens than in non-muscle-invasive bladder cancer (NMIBC) specimens. Strong LPA1 expression was evident on cell membranes of MIBC specimens. Cancer-specific survival rate was predominantly lower in LPA1 positive group. T24 cell invasion was increased by LPA treatment, and invasiveness was decreased by LPA1-siRNA or LPA1 inhibitor. LPA treatment increased Rho kinase1 (ROCK1) expression and phosphorylation of MLC, and induced morphological changes including lamellipodia formation and cell rounding. CONCLUSIONS Our results indicated that LPA1 expression was increased in bladder cancer with highly invasive potential. Furthermore, LPA signaling via LPA1 activation promoted bladder cancer invasion. Analyzing LPA1 expression might be useful for planning of bladder cancer treatment, and LPA1 can be a new therapeutic target for highly invasive bladder cancer. © 2017FiguresReferencesRelatedDetails Volume 197Issue 4SApril 2017Page: e638 Advertisement Copyright & Permissions© 2017MetricsAuthor Information masao kataoka More articles by this author tomoyuki koguchi More articles by this author Michihiro Yabe More articles by this author Yuuichi Satoh More articles by this author Nobuhiro Haga More articles by this author Kei Ishibashi More articles by this author Ken Aikawa More articles by this author Yoshiyuki Kojima More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...