Nutritional evaluation and functional properties of the antioxidant polypeptide from Zanthoxylum bungeanum Maxim seeds kernel protein hydrolysate

Antioxidant polypeptides of Zanthoxylum bungeanum Maxim seeds kernel (ZBMSK) were investigated based on molecular weight distribution, amino acid composition, and functional properties. The polypeptide had a molecular weight distribution of 1026 similar to 2249Da and 4926 similar to 6398Da, and was rich in glutamic acid, arginine, aspartic acid, and leucine, but poor in cysteine and tryptophan. Total amino acids were 56.42g/100g, and lysine was the first limiting amino acid. The polypeptide showed excellent solubility, viscosity, emulsifying properties, and foam expansion compared with its original protein. In addition, the polypeptide exhibited favorable reduction power and scavenging capacities for DPPH radicals with an IC50 value of 8.86mg/mL. These results suggest that the polypeptide derived from ZBMSK has potential future application among functional foods, and may be a promising source of natural antioxidants. RESUMENSe investigaron los polipeptidos antioxidantes de semillas de Zanthoxylum bungeanum (ZBMSK) en base a la distribucion del peso molecular, la composicion de aminoacidos y las propiedades funcionales. Los polipeptidos presentaban un peso molecular de 1026 similar to 2249 Da y 4926 similar to 6398 Da, ademas eran ricos en acido glutamico, arginina, acido aspartico y leucina, aunque pobres en cisteina y triptofano. El total de aminoacidos fue de 56,42 g/100 g y la lisina fue el primer aminoacido de limitacion. Los polipeptidos mostraron una excelente solubilidad, viscosidad, propiedades emulsionantes y expansion de espuma en comparacion con su proteina original. Ademas, los polipeptidos exhibieron un poder reductor favorable y capacidades de barrido de radicales DPPH con un valor IC50 de 8,86 mg/mL. Estos resultados sugieren que los polipeptidos derivaros de ZBMSK tienen una futura aplicacion potencial entre los alimentos funcionales, ademas podrian ser una prometedora fuente de antioxidantes naturales.