Multi-center evaluation of one commercial and 12 in-house real-time PCR assays for detection of Mycoplasma pneumoniae
Diagnostic Microbiology and Infectious Disease(2017)
Abstract
Detection of Mycoplasma pneumoniae by real-time PCR is not yet standardized across laboratories. We have implemented a standardization protocol to compare the performance of thirteen commercial and in-house approaches. Despite differences on threshold values of samples, all assays were able to detect at least 20M. pneumoniae genomes per reaction.
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Key words
Mycoplasma pneumoniae,Detection,Real-time PCR,Multi-center evaluation
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