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Multi-center evaluation of one commercial and 12 in-house real-time PCR assays for detection of Mycoplasma pneumoniae

Roger Dumke,Alvaro J. Benitez,Victoria J. Chalker,Karolina Gullsby,Birgit Henrich,Carlos Hidalgo-Grass,Theo Hoogenboezem,Darja Keše,Katherine Loens,Jolanda Maaskant,Ayelet Michael-Gayego,Allon E. Moses,Ran Nir-Paz,Suzan D. Pas,Sabine Pereyre,Randi Føns Petersen,Meike Rosenblatt,Annemarie M. C. van Rossum,Søren A. Uldum,Wendy W.J. Unger,D. Ursi,Jonas M. Winchell,Cécile Bébéar

Diagnostic Microbiology and Infectious Disease（2017）

Cited 5|Views52
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Abstract
Detection of Mycoplasma pneumoniae by real-time PCR is not yet standardized across laboratories. We have implemented a standardization protocol to compare the performance of thirteen commercial and in-house approaches. Despite differences on threshold values of samples, all assays were able to detect at least 20M. pneumoniae genomes per reaction.
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Key words
Mycoplasma pneumoniae,Detection,Real-time PCR,Multi-center evaluation
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