A novel colony PCR assay format using SYBR green technology
Pathology(2017)
摘要
Real-time PCR (RT-PCR) assays using SYBR Green as an indicator of product amplification are thwarted by potential for poor specificity. We have validated a novel format for testing isolated colonies from plate cultures that is highly specific, uses 75% less reagents and circumvents preliminary extraction.
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关键词
pcr assay,sybr
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