Relations Of T-Cell And B-Cell Area Ratios And The Number Of Natural Killer Cells (Nk Cells) In Lymph Nodes (Lns) To Outcomes In Patients With Stage Ii Colon Cancer.

618 Background: In stage II colon cancer, patients with many retrieved LNs have good outcomes. We previously reported that the longest diameter of retrieved LNs correlates with the number of LNs and can be used as a prognostic factor (Int J Colorectal Dis 2015). LNs can be divided into three main regions, cortex, paracortex, and medulla, and consist mainly of T cells, B cells, NK cells, and histiocytes. We studied the relations of the T-cell, B-cell area ratios, and the number of NK cells in longest-diameter LNs to the number of retrieved LNs and outcomes. Methods: The subjects were 320 patients with stage II colon cancer who underwent curative resection. One LN with the maximum long-axis diameter was selected, and was immunostained with CD3 as a T-cell marker, CD20 as a B-cell marker, and CD56 as an NK-cell marker. Positive area ratio was determined by image analysis. The number of CD56-positive cells were examined and the mean number per 0.093 mm2was calculated. The relations were evaluated with the use...