Abstract P40. Platelet Rich Plasma Augments Adipose-Derived Stem Cell Growth and Differentiation

Purpose: Adipose-derived stem cells (ASCs) are a powerful therapeutic and research tool. Unfortunately, ASCs grow slowly and are sensitive to culture conditions, thereby limiting their therapeutic application. Platelet rich plasma (PRP) has recently been used to improve outcomes in regenerative medicine. We hypothesize that the addition of PRP during ASC isolation and culture may alleviate limitations on ASC growth. Methods: Fresh non-activated PRP (fPRP), flash frozen PRP (ffPRP), and one month cryopreserved PRP (cPRP) were added after stromal vascular fraction isolation. A series of PRP feeding protocols were tested throughout 7 days of growth using the Cyquant Cell Proliferation Assay Kit (Life Technologies) and compared to non-PRP controls. Osteogenic capacity with and without PRP was also tested. Results: fPRP and cPRP groups demonstrated reduced cell adherence after isolation compared to non-PRP control groups (n=4/group, p<0.001). In contrast, ffPRP groups showed similar or better adherence than non-PRP control groups. When feeding protocols were compared after 7 days of growth, a single administration of either fPRP or ffPRP resulted in significantly larger population sizes than all other protocols (p<0.01). One of the 4 patients tested showed spontaneous osteogenesis in the presence of ffPRP. Conclusion: We demonstrated that the addition of PRP significantly improves ASC growth and likely therapeutic application. The optimal combination to augment ASC adherence and growth is a single dose of ffPRP without additional feeding or cell manipulation. However, PRP may induce osteogenesis in specific patients and additional studies into the molecular mechanism of PRP regulation of ASCs are needed.