Non-mucoid P. aeruginosa aiming to a safe production of protease and lipase

ABSTRACT: Being an opportunistic pathogen, P. aeruginosa has a limit contribution in biotechnology applications. H 2 O 2 can induce mucoid conversion in P. aeruginosa . Five non-mucoid P. aeruginosa strains were investigated in this study. After applying H 2 O 2 to these non-mucoid strains two strains were converted to be mucoid. The stable non-mucoid strains were investigated for their ability to produce protease and lipase. Agar well diffusion was used for detecting the protease and lipases activity. Additionally Zymogram was used for in gel determination of the protease activity. Spectrophotometrically, proteases and lipases activities were determined using casein and p -nitrophenyl palmitate as substrates respectively. The apparent activity for each enzyme identified as units/ml. Protease activity was 428 units/mL while lipase activity was 21 units/mL. This study proves the ability of isolating stable non-mucoid P. aeruginosa strain able to produce of both protease and lipase. The H 2 O 2 mucoid-non-mucoid test described in this study should be used in the regular P. aeruginosa identification. We recommended using the non-mucoid P. aeruginosa strain isolated in this study in different biotechnological applications. Keywords: P. aeruginosa , Alginate, H 2 O 2 , protease, lipase