Monitoring Small Molecule And G-Quadruplex Interactions And Kinetics Using Single Molecule Fret

Telomestatin and oxazole telomestatin derivatives (OTD) are small molecules stabilizing G-quadruplex (GQ) structures and are prominent due to their anti-cancer drug potential. Despite the observation of enhanced thermodynamic stability imparted by such small molecules on GQ, the underlying dynamics of small molecule-GQ interactions are not known. To have a better understanding of these interactions we employed single molecule Forster resonance energy transfer (smFRET) to study the system, where we utilized a Cy5-labeled OTD (L1Cy5-7OTD).