Enhanced topical delivery of fish scale collagen employing negatively surface-modified nanoliposome

In this study, we investigated the effect of fish scale collage (FSC) on type I procollagen expression in cultured human dermal fibroblasts using real-time polymerase chain reaction and ELISA, under normal and UV exposure condition. Also, FSC encapsulated negatively surface modified nanoliposome (NL surf mod ) was fabricated, and the physicochemical characteristics of the liposomes and skin permeation properties were evaluated. FSC increased type I procollagen mRNA and protein expression in human dermal fibroblasts. The mean particle size, zeta potential value and encapsulation efficiency of the NL surf mod were about 175 nm, −44 mV and 11%, respectively. The skin localization effect was enhanced by NL surf mod . Conclusively, FSC is able to elevate the type I procollagen expression in human dermal fibroblasts and FSC-loaded NL surf mod would be a good candidate for the topical delivery of FSC.