PP - EFFECTS OF PHOTODYNAMIC THERAPY MEDIATED BY NANOEMULSION CONTAINING CHLORO-ALUMINUM PHTHALOCYANINE

The aim of the present work was to evaluate the effects of local application of nanoemulsion chloro-aluminum phthalocyanine (AlClFc) with a specific type of light on human gingiva and analyze by means of immunohistochemistry the expression of VEGF in a split-mouth model. Eight healthy volunteers with clinical indication for extraction were included in the study. Seven days before the extraction, 40μl of nanoemulsion containing AlClPc 5μM was injected into gingival tissue followed by irradiation with diode laser (660nm, 7J/cm2). Tissue specimens were removed seven days after PDT and divided into two groups for histological and immunohistochemical analysis. Patients were monitored at days 0, 7, 14 and 30 to assess adverse effects of the therapy. Areas of edema, vascular congestion, and intense vascularization were viewed in gingival samples that received PDT. Dystrophic calcification was observed in the subepithelial region of the test group. VEGF showed moderate to strong immunostaining in specimens from the test group. The therapy was well tolerated by all patients. Taken together, the results showed that the protocol used in this study mediated by nanoemulsion containing AlClPc is safe for clinical application in gingival tissue and suggests that VEGF is increased after PDT application.