P1.02-079 DNA Ploidy, CPA4 and Rel B Expression in Non-Small Cell Lung Cancer: Correlation with Clinicopathologic Parameter: Topic: Other Mutations in Thoracic Malignancies

The aim of this study was to evaluate the expression of CPA4 a member of carboxypeptidase family and Rel B a member of nuclear transcription factor Kappa B family in imprints of resected NSCLC and correlate these expressions with DNA ploidy and classical prognostic factors. A total of 45 smears of patients who underwent surgical treatment for NSCLC were examined immunocytochemicaly for the expression of CPA4 and Rel B. Imprint smears also were stained using the Feulgen procedure in order to evaluate DNA ploidy in the same cases. Thirty two (71,1%) of the tumors were classified as aneuploid. CPA4 positive expression was observed in 18 (40%) and Rel B in 21 (46,7%) of the tumors. We found a significant relationship between DNA ploidy and grade (p=0,005). CPA4 and Rel B expression correlated with grade (p<0,0001 for both) and also with nodal status (p=0,004 and p=0,017, respectively). Cox regression multivariable analysis demonstrated that CPA4 and Rel B expression were independent prognostic factors. The mean DNA index was higher for tumors with negative expression of CPA4 and Rel B (P=0,045 and p=0,025 respectively) DNA aneuploidy correlates with poor and moderately differentiated tumors. CPA4 and Rel B positive expression is in relation to well differentiated carcinomas and nodal status