SPECIFIC KNOCKDOWN OF EGFR AND PLK1 GENE EXPRESSION IN U87 GBM CELLS WITH RADIOFREQUENCY ENERGY (RFE) DEVICE

Every molecule contains a unique electromagnetic radiofrequency energy (RFE) potential. Using superconducting quantum interference device (SQUID) technology, we have developed a technique to specifically measure the RFE of a given molecule, and then expose living tissues to the specific and precise oscillating magnetic RFE field of that molecule. We discovered that exposure of a molecule’s unique RFE through a transmitting electromagnetic coil device can specifically impact biological systems as if the authentic molecule were present. We have recently acquired the RFE signals of siRNA oligonucleotide molecules. Here, we demonstrate for the first time that disruption of signaling pathway using RFE can significantly inhibit target mRNA and protein expression. EGFR and PLK1 blockade by RFE caused a 30-70% reduction of mRNA expression and ~40% protein expression vs control treated cells. Moreover, using RNA microarray and protein array we validated specificity of cellular EGFR knockdown. Finally, we demonstrated that RFE-mediated knockdown of EGFR and PLK1 was associated with decreased U87 GBM cell proliferation. These are the first results to demonstrate that specific RFE signals can be used to knock down specific cellular genes that can impact key pathways associated with cancer signaling.