Development Of A Novel Technique To Isolate And Characterize Mouse Podocytes

Podocytes play a key role in the selective filter that underlies kidney function. The loss of mature podocytes is involved in chronic kidney disease progression. The mechanisms of podocyte injury, leading to proteinuria are still unclear due to lack of consistent and validated methods to study primary podocytes. In vitro system, glomerular outgrowth and human immortalized cell line (HIC) have their merits; however, these cells fail to express podocyte specific markers and variations in culture protocols challenge their utility. Hence, an isolation method is required to define potential mechanism involved in disease progression.