CORALINA: a universal method for the generation of gRNA libraries for CRISPR-based screening
BMC Genomics(2016)
Abstract
The bacterial CRISPR system is fast becoming the most popular genetic and epigenetic engineering tool due to its universal applicability and adaptability. The desire to deploy CRISPR-based methods in a large variety of species and contexts has created an urgent need for the development of easy, time- and cost-effective methods enabling large-scale screening approaches.
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Key words
gRNA library,Genome-wide,Cas9,Genetic engineering,Epigenetic engineering,Elongated protospacer,Epigenome editing
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