Investigation of cellular response to covalent immobilization of peptide and hydrophobic attachment of peptide amphiphiles on substrates

•Two types of surface were designed.•In one of them, RGD peptide was covalently immobilized on silicon surfaces.•The lipidated version of RGD peptide was hydrophobically attached on alkylated surfaces.•Cellular response of all modified surfaces was examined.•Hydrophobically attached lipidated RGD peptide increased cellular response significantly.