P094 Human leukocyte antigen characterization of a Kumeyaay ethnic group community in Baja California, Mexico

The Kumeyaay Nation is part of the Yuman cultural complex.It is disseminated along the states of Arizona and California in the USA, as well as the states of Baja California and Sonora in Mexico.San Jose de la Zorra is one of the four native Kumeyaay communities in Baja California, with a population of around 200 people.The HLA class I and II profile has not been previously explored for them.The aim of this study was to obtain high-resolution HLA class I and class II typing for a group of Kumeyaay persons from San Jose de la Zorra. Methods The study was performed in 35 individuals belonging to nine families.All of them agreed to participate and sign an informed consent.DNA was extracted from peripheral blood using Maxwell 16.HLA high-resolution typing was performed through NGS using the TruSight HLA kit and the Miseq, Illumina.The sequence analysis was done with the Assign software.Allele and haplotype frequencies were calculated from “less related individual” (HLA-net.eu). Results A total of 28 different haplotypes were identified.Nine of them were from a European or African origin.The allele profile found in Amerindian haplotypes was very restricted, with two or three alleles in each locus adding around 50–60% of the total.The most frequent alleles in each locus were:A ∗ 02:06, ∗ 68:01, ∗ 31:01; B ∗ 40:02, ∗ 27:05, ∗ 35:01; C ∗ 03:04, ∗ 02:02, ∗ 04:01, DRB1 ∗ 14:02, ∗ 08:02, ∗ 16:02; DQB1 ∗ 03:01, ∗ 04:02 and DPB1 ∗ 04:01, ∗ 04:02. Conclusions A very restricted HLA class I and II allele profile was found in Kumeyaay, similar to what has been described for other Amerindian populations. The allele profile is very different from the one found in Seris, that also live in the northwest of Mexico, but belong to a different linguistic trunk. For example in Seris B ∗ 27:05 u0026 DRB1 ∗ 14:02 are almost absent. The presence of 25% of non-Amerindian haplotypes can be explained, partially, as the result of admixture with Spaniard and English migrants that arrived at the peninsula in the 1800s.