Comprehensive Mutation Analysis In Nrg Oncology/Rtog 9802: A Phase Iii Study Of Rt Vs Rt Plus Pcv In High-Risk Low-Grade Gliomas (Lggs).

2017Background: This study sought to examine the prognostic significance of mutations within IDH1/2, ATRX, CIC, FUBP1, and the TERT promoter in a prospective phase III study of high-risk LGG using multivariate analyses (MVAs). Methods: IDH mutation status was determined by immunohistochemistry and/or deep sequencing. A custom Ion AmpliSeq DNA panel was used for mutation analysis; TERT promoter mutations were detected by Sanger sequencing. Progression-free survival (PFS) and overall survival (OS) were estimated using the Kaplan-Meier method. Hazard ratios (HRs) were calculated and tested using the Cox proportional hazard model and the log-rank test. MVAs were performed incorporating age, treatment, surgery, histology, and performance status as covariates. Results: Observed mutations, by frequencywere 74% (85/115) within IDH1/2, TERT promoter in 40% (32/80), CIC in 25% (17/69), ATRX in 24% (16/67), and FUBP1 in 10% (7/69). In the univariate analysis (UVA) on OS, IDH1/2 (HR = 0.40; p u003c 0.001), CIC (HR = 0.40...