In-vitro Antagonistic Potential of Different Fungi Against Fusarium oxysporum f. sp. Capsici

The current research was conducted in Lab. to assess an antagonistic effect of various fungi against Fusarium oxysporum f. sp. capsici . In the present research, each treatment ( Trichoderma viride, T. harzianum and T. Koningii ) with three concentrations viz. 1×10 5, 1×10 6, 1×10 7 spores/ml and three replications were assessed through well method in Phytobacteriology lab, Department of Plant Pathology, University of Agriculture, Faisalabad. Spore concentration was adjusted with the help of haemocytometer and data was recorded after 5 days of inoculation. T. viride, T. harzianum and T. Koningii expressed 1.14, 1.31 and 1.57 cm colony growth in 9cm petri plate respectively. T. viride exhibited maximum inhibition of Fusarium oxysporum f. sp. capsici after comparison of mean colony growth. The interaction between Treatment and Concentration (T×C) showed that 1×10 5 conidia/ml expressed 1.44, 1.63, 1.88 and 6.14 cm colony growth of Fusarium oxysporum f. sp. capsici in petri plat through poisoned food technique by T. viride, T. harzianum, T. Koningii and control as compared to 1×10 6 conidia/ml showed 1.11 to 1.60 cm and 1×10 7 conidia/ml of T. Viride exhibited minimum colony growth (0.881) as compared to T. harzianum (0.96) and T. Koningii (1.22) cm respectively. Similarly, the interaction between Treatment and Days (T×D) expressed maximum (1.70, 1.96, 2.20 and 6.47) cm colony growth by fungus after tenth day as compared to (1.13, 1.33, 1.56 and 6.27) cm after seventh day and T. Koningii (0.94), T. harzianum (0.63) and T. Viride (0.60) cm after fourth day respectively while interaction between T×C×D expressed that 1×10 7 conidia/ml of T. viride (0.36, 0.8, 1.46), T. harzianum (0.4, 1.0, 1.6) and T. koningii (0.6, 1.26, 1.8) cm exhibited minimum colony growth after day four, seventh and tenth as compared to 1×10 6 conidia/ml (0.56, 0.60, 0.86, 5.70, 1.16, 1.40, 1.60, 6.27, 1.60, 2.00, 2.33 and 6.47) cm and 1×10 5 conidia/ml showed maximum colony growth (0.86, 1.03, 1.36, 5.70, 1.43, 1.60, 1.80, 6.26, 2.03, 2.26, 2.46 and 6.47) cm at fourth, seventh and tenth days respectively. It was concluded that T. viride inhibited the colony growth of Fusarium oxysporum f. sp. capsici most effectively at concentration 1×10 7 conidia/ml.