Inconsistencies in C. elegans behavioural annotation

High quality behavioural annotation is a key component to link genes to behaviour, yet relatively little attention has been paid to check the consistency of various automated methods and expert judgement. In this paper we investigate the consistency of annotation for the ‘Omega turn’ of C. elegans, which is a frequently used behavioural assay for this animal. First the output of four Omega detection algorithms are examined for the same data set, and shown to have relative low consistency, with F-scores around 0.5. Consistency of expert annotation is then analysed, based on an online survey combining two methods: participants judged a fixed set of predetermined clips; and an adaptive psychophysical procedure was used to estimate individualu0027s threshold for Omega turn detection. This survey also revealed a substantial lack of consistency in decisions and thresholds. Such inconsistency makes cross-publication comparison difficult and raises issues of reproducibility.