An Arabidopsis cyclin promoter region is active in transgenic maize plants
Cereal Research Communications(1999)
摘要
We have produced transgenic maize carrying Arabidopsis cyclin promoter and β-glucuronidase chimeric fusion (cycBl;lAt::GUS) by direct DNA uptake into embryogenic cell suspension protoplasts to study the activity of this cyclin promoter in a heterologous cell system. The transgenic maize lines exhibiting different levels of reporter gene expression show that regulatory elements from cycB1;1At gene as a dicot promoter can function in maize. Expression patterns revealed by fluorimetric, as well as, histochemical GUS reporter enzymatic assays indicated cell division-dependent expression of the GUS gene driven by this cycBl;At;l promoter with elevated activity in proliferating cells and meristems. Treatment of tissues with inhibitors of mitosis increased the activity of cycB1;1At promoter, that suggests a link between mitotic phase of cell division and activity of cycBl;lAt regulatory elements in transgenic maize.
更多查看译文
关键词
transgenic plants,cyclin promoter,gene expression,cell cycle,maize
AI 理解论文
溯源树
样例
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要