In situ immobilization of lysine decarboxylase on a biopolymer by fusion with phasin: Immobilization of CadA on intracellular PHA

•An in situ immobilization system for enzymatic cadaverine production was developed.•CadA was immobilized on poly (3-hydroxybutyrate) (P(3HB)) biopolymer granules.•Increased P(3HB) production was observed with phasin-fused CadA.•The thermal stability increased with immobilization.•Repetitive reactions of the CadA–P(3HB) complex were observed.