Sialylation levels influence oligosaccharide quantitation: Analyzing response variability using high-pH anion-exchange chromaltogrophy and pulsed amperometric detection

Chandrashekar Ganesa, Brian W. Granda,Robert J. Mattaliano

BIOPHARM INTERNATIONAL-THE APPLIED TECHNOLOGIES OF BIOPHARMACEUTICAL DEVELOPMENT（2003）

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摘要

Oligosaccharide structures and the degree of sialylation can cause variability among detection cells when using high-pH anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) technology for oligosaccharide characterization. That variability can affect quantitative results used to determine batch-to-batch consistency.

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