Recovery Of Cellulolytic Enzymes From Wheat-Straw Hydrolysis Broth With Dioctyl Sulphosuccinate/Isooctane Reverse Micelles

Hydrolysis of wheat straw was carried out with cellulases and hemicellulases from Trichoderma reesei QM 9414 cultures, The recovery of cellulolytic enzymes from hydrolysis broth was assayed through a forward transfer to dioctyl sulphosuccinate (AOT)/iso-octane reverse micelles and a backward transfer to a fresh aqueous medium, Neither reducing sugars nor DNA were recovered, but we detected beta-glucosidase, beta-xylosidase and xylanase activities in the final aqueous medium, We studied the dependence of protein recovery on the pH of hydrolysis broth, the AOT concentration, and the w(Oi) (molar ratio of water to AOT) of the reverse micelles as well as the temperature of both transfers and the ionic strength of the aqueous medium in the backward transfer, High temperature increased the recovery of enzymes, whereas the optimum pH varied for each enzyme. Moreover, the water content of the organic medium was titrated in each experiment, The hydrodynamic radius of the reverse micelles was higher when protein was solubilized. Under optimum transfer conditions, 21.5% of beta-xylosidase and 7.5% of beta-glucosidase activity was recovered.