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Wolbachia spp. interferes with molecular detection of Ehrlichia canis and Anaplasma platys in microfilaremic dogs.

Marcia de Souza Xavier, C. N. Fonseca,Renata Fernandes Ferreira,Tatiana Xavier de Castro,Felipe Piedade Gonçalves Neves,André Victor Barbosa,A.M.F. Cerqueira,Nádia Regina Pereira Almosny

ARQUIVO BRASILEIRO DE MEDICINA VETERINARIA E ZOOTECNIA（2015）

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Abstract
This report aimed to study the interference in molecular testing for Ehrlichia canis and Anaplasma platys in blood of 155 dogs from the coastal region of Rio de Janeiro. Five Anaplasmataceae positive samples but negative for E. canis and A. platys, from microfilaremic animals, were chosen for sequencing. These sequences, when compared to Gen et Bank database, showed 88% to 100% similarity with Wolbachia spp. denoting an interference in the detection of DNA from other members of Anaplasmataceae, possibly due to a high concentration of Wolbachia spp. DNA.
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dogs,PCR,ehrlichiosis,sequencing,microfilaremic
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