Quantitative analysis of ursodeoxycholic acid and related compounds in drug substances and dosage forms by HPTLC with scanning densitometry
JPC-JOURNAL OF PLANAR CHROMATOGRAPHY-MODERN TLC(1997)
Abstract
An high-performance thin-layer chromatographic (HPTLC) method on silica gel, with chloroform - acetone - acetic acid (70 + 20 + 1, v/v) as mobile phase, has been used for the qualitative and quantitative determination and purity control of ursodeoxycholic acid in bulk samples and pharmaceutical dosage forms. Detection by post- chromatographic color reaction was achieved by spraying with 20% m/v phosphomolybdic acid in 95% ethanol and then heating the plate at 105 degrees C for 5 min. Spots were quantified by scanning at lambda = 710 nn. The potential impurities are well separated from ursodeoxycholic acid and from each other with the exception of chenodeoxycholic and deoxycholic acids which overlap. Detection limits at a signal-to-noise ratio of 2:1 were below 15 ng per spot, Linearity, reproducibility, and percentage recovery of ursodeoxycholic acid from dosage forms are reported. Reliable results were obtained.
MoreTranslated text
Key words
ursodeoxycholic acid and related compounds,HPTLC,densitometry
AI Read Science
Must-Reading Tree
Example
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
Generate MRT to find the research sequence of this paper
Chat Paper
Summary is being generated by the instructions you defined