Quantifying ROS levels using CM-H2DCFDA and HyPer.

Monika Oparka,Jarosław Walczak,Dominika Malinska,Lisanne M.P.E. van Oppen,Joanna Szczepanowska,Werner J.H. Koopman,Mariusz R. Wieckowski

Methods（2016）

引用 129|浏览38

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摘要

•Two strategies to monitor ROS using CM-H2DCFDA and HyPer are described.•Protocols employing microscopic and fluorometric techniques are presented.•Fluorescent protein-based sensor “HyPer” is a specific H2O2-biosensor.

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关键词

Reactive oxygen species,Hydrogen peroxide,CM-H2DCFDA,HyPer,SypHer

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