Magnetic isotope of magnesium accelerates ATP hydrolysis catalyzed by myosin

In this paper, we present the results of experimental studies on the influence of different magnesium isotopes, magnetic 25 Mg and nonmagnetic 24 Mg or 26 Mg, on ATP-hydrolytic activity of the isolated myosin subfragment-1. The reaction rate in the presence of magnetic 25 Mg isotope turned out to be 2.0–2.5 times higher than that using non-magnetic 24 Mg or 26 Mg isotopes. In absence of the enzyme, as at spontaneous ATP hydrolysis in aqueous solution, no magnetic isotope effect was observed. Thus, a significant catalytic effect of the magnetic 25 Mg isotope (nuclear spin catalysis) was discovered in the enzymatic hydrolysis of ATP.