High-throughput biochemical profiling reveals Cas9 off-target binding and unbinding heterogeneity
Proceedings of the National Academy of Sciences(2016)
摘要
The bacterial adaptive immune system CRISPR-Cas9 has been appropriated as a versatile tool for editing genomes, controlling gene expression, and visualizing genetic loci[1][1]. To analyze Cas9’s ability to bind DNA rapidly and specifically, we measured the kinetics of catalytically dead Cas9 (
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