A method for the quantitation of homologous endopeptidases AlpA and AlpB from Lysobacter sp. XL1

Anna Petrovna Karatovskaya,Natalia V. Rudenko,I. M. Tsfasman, Ksenya A. Guseva,Aleksandr Laman,Khanafi Magometovich Boziev,F. A. Brovko,Natalia V. Vasilyeva

Process Biochemistry（2016）

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摘要

•Monoclonal antibodies to homologous proteases AlpA and AlpB were produced.•A sandwich immunoassay for the quantitation of enzymes of Lysobacter sp. was developed.•The detection ranges were 0.2–3.1 and 3–100ng/ml for AlpA and AlpB, respectively.

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关键词

Lysobacter,Endopeptidases,Preproproteins,Monoclonal antibodies,Monoclonal antibodies to peptides,Sandwich enzyme immunoassay

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