Flow cytometric enumeration of bacterial in the coral surface mucus layer

Yvan Bettarel,Mai Chi Thanh,Got Patrice,Adingra Antoinette,Kouadio-Ngbesso Nadège,Van Ngoc Bui,Bouvier Thierry

Journal of Microbiological Methods（2016）

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摘要

The direct counts of bacteria inhabiting coral mucus were performed by flow cytometry testing four fluorescent dyes (SYBR®Green I, HCS, TOPRO®3, SYTO®62) with three different scleractinian species. Results obtained with SYTO62 were the most reliable based on the comparison with standardized epifluorescence counts and the resolution of cytograms.

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关键词

Bacteria,Corals,Fluorescent dyes,Mucus,Epibionts,Microscopy

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