Effect of Renal Transplantation on Selected Biomarkers of Oxidative Stress and Oxidative Modification of Biomolecules

It was demonstrated that oxidative stress plays important role in patients with end-stage renal disease. The aim of this study was to evaluate the effect of transplantation on oxidative stress level in patients compared to the patients on dialysis. 49 patients who underwent a renal transplantation and 63 patients on dialysis (with CKD) were included in study. Kidney transplant patients had good graft function (MDRD = 1.078 ml/s per 1.73m2, serum creatinine levels 99.18 micromol/l). Plasma levels of markers of oxidative damage to biomolecules were determined – advanced oxidation protein products (AOPP), nitrotyrosines, lipoperoxides (LP), 8-isoprostanes (8-Iso) and 8-oxo-guanosine (8-oxoG). In addition activities of antioxidative enzymes (SOD, GPx, CAT) and plasma total antioxidant status (TEAC) were measured. We observed in transplanted patients compared to dialyzed ones significant improvement in oxidative modifications of lipids, proteins and DNA, represented by decreased LP level by 33.45%, 8-Iso by 55.87%, AOPP by 29.24% and 8-oxoG by 65.58%. Although TEAC was not changed, SOD and GPx activities were significantly increased by 5.95% and 14.82% resp. Following our results we can conclude that transplantation has improving effect on oxidative damage to biomolecules and antioxidant systems of organism in kidney diseases.