Improved Clinical Scale MSC Isolation and Expansion with Matrix- and Xeno-free Medium from Primary Tissue
CYTOTHERAPY(2016)
摘要
Background: The need for a xeno-free and GMP conform production of clinical scale mesenchymal stem cells (MSCs) is rising. A number of clinical PhII/III trials are registered and the use of fetal calf serum (FCS) is undesirable with regard to adverse events and lot-to-lot variations. MSC-Brew GMP Medium supports the isolation and expansion of MSCs from various tissues. The medium is xeno-free and can be used without additional coating of cell culture vessels. We compared MSC-Brew GMP Medium with other xeno-free MSC media as well as FCS- and platelet lysate (PL)-containing formulations.
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关键词
Mesenchymal Stem Cells
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