Improving productivity of a pyrrolnitrin producer

Objective To improve the productivity of a pyrrolnitrin producer by using a novel approach.Method Introducing aminoglycoside antibiotic resistant mutation into a pyrrolnitrin producer to increase pyrrolnitrin productivity.Results The mutants from the NB agar containing streptomycin,hyromycin,geneticin and gentamycin of 3 fold and 10 fold MIC were obtained.The mutants with 5 fold and 10 fold pyrrolnitrin productivity were selected.The results indicated that streptomycin resistance was most effective in improving pyrrolnitrin productivity although streptomycin had very high MIC.Geneticin and gentamycin with lower MICs than streptomycin,was less effective in improving pyrrlintrin productivity.Hygromycin was also effective in improving pyrrolnitrin productivity to some extent.Conclusion Introducing aminoglycoside antibiotic resistance mutation is effective in improving pyrrolnitrin productivity.