Impact of Ratio of Anticoagulant/Blood on Examining PTA in Patients with Hepatitis

Objective To investigate the impact of ratio of anticoagulant/blood on examining prothrombin activity (PTA) in patients with hepatitis. Methods Blood samples were taken from 36 cases with hepatitis and PTA was examined under different experimental condition, in which anticoagulant and the blood were mixed in ratio 1:9 and 1:6. Results In all patients with clinical symptom of hepatitis,significant difference with PTA were observed when comparing the group at the ratio of 1:9 with that of 1:6. Comparing two groups of ration 1:9 and 1:6, the PTA were 95 ± 15.2 and 54 ± 5.6, respectively, in mild chronic hepatitis group, and 54±6.1,37±3.5, respectively, in moderate and severe chronic hepatitis group, while the PTA were 28± 6.4,18 ±7.2,respectively,in grave hepatitis groups. Conclusion Under the experimental condition,in which the ratio of anticoagulant/ blood is 1:9, the sensitivity of PTA test is higher, and the PTA value is more coincidental with clinical type of hepatitis.