Studying on Ciprofloxacin killing of Neisseria Gonorrhoeae by Flow Cytometry
China Public Health(2001)
Abstract
Objective Developing a rapid assay of susceptibility of neisseria gonorrhoeae(Ng)to ciprofloxacin(CIPF).Methods After treated with CIPF,12 strains Ng was detected by flow cytometry,and these data were analysed by double-parameters.Results The mean fluorescence channel(FL3 log )of living Ng and dead Ng killed by heat was 0 43 and 75 7 respectively.We determined 12 strains Ng incubatd with 2μg/ml CIPF for 2h at 37℃ by flow cytometry,and the results were that the rate of dead bacteria of 6 strains CIPF resistant Ng and 6 strains CIPF susceptible Ng were(14 1±4 5)% AND (40 1±5 8)% respectively,which were significantly differnet(P0 001).The mean fluorescance channel of dead bacteria of 6 strains CIPF resistact Ng and 6 strains CIPE susceptible Ng were(14 1±4 5) and (40 1±5 8)respectively,which were also significiently different(P0 05).Conclusion Rapid assay Ng susceptibility to CIPF can be achieved by flow cytometry.
MoreTranslated text
Key words
Neisseria gonorrhoeae
AI Read Science
Must-Reading Tree
Example
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
Generate MRT to find the research sequence of this paper
Chat Paper
Summary is being generated by the instructions you defined