超高效液相色谱串联质谱测定乳及乳制品中AFM1、AFM2
Food Research and Development(2014)
Abstract
利用免疫亲和萃取结合超高效液相色谱-串联四极杆质谱技术(UPLC-MS/MS)建立了乳及乳制品中黄曲霉毒素M1和M2的提取、分离、确证与定量方法。乙腈和水提取样品中的黄曲霉毒素,乙腈二次去蛋白后用免疫亲和柱净化,采用Waters Acquity UPLC R HSS T3(2.1×100 mm,1.7μm)色谱柱实现分离,多反应监测(MRM)模式下以外标法定量。结果表明:黄曲霉毒素M1和M2在0.1μg/L~50μg/L范围内线性关系良好(r﹥0.9985);检出限为0.029μg/L~0.047μg/L(S/N=3);2种黄曲霉毒素各添加水平在鲜牛奶、奶粉和奶油中的回收率为在69.56%~88.43%之间;相对标准偏差为3.69%~8.74%。
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Key words
aflatoxin m1,dairy products,mass spectrometry,ultra-high,chromatography-tandem
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