Effect of pulsed electromagnetic fields produced by different frequencies on bone metabolism markers in osteoprotegerin gene knock-out mice

Objective To explore the optimal frequency of pulsed electromagnetic fields for the treatment of osteoporosis,by using different frequencies of pulsed electromagnetic fields to treat osteoprotegerin(OPG) knock-out mouse model Methods Fifteen male and fifteen female 8-week OPG knock-out mice were randomly divided into 3 groups: Group A,Group B,and Group C.Each group had 10 mice.Group A was blank control group with no intervention.Mice in Group B received PEMFs treatment at 8 Hz frequency with 4.0 mT intensity,20 min per day,for 30 days.Mice in Group C received PEMFs treatment at 32 Hz frequency with 4.0 mT intensity,20 min per day,for 30 days.Tartrate-resistant acid phosphatase 5b and serum bone γ carboxyglutamic acid of all rats in each group were measured using ELISA before the treatment and 30 days after the treatment.Results The concentrations of tartrate-resistant acid phosphatase 5b and serum bone γ carboxyglutamic acid in Group B and C after the treatment were significantly different with those in Group A(P0.05).Whereas the bone metabolism markers in Group B before or after the treatment had no significant difference compared with those in Group C(P0.05).Conclusion The treatment of pulsed electromagnetic fields for osteoprotegerin gene knock-out mice is really effective.Both the frequency of 8 HZ and 32 HZ can produce the same therapeutic effect.